Hazelnut allergome overview and Cor a gRNAs identification

BMC Plant Biology

Table 2 Estimation of Non synonymous and synonymous substitutions mean dissimilarity (ω = dN /dS ) for each Corylus TLP gene family and phylogentic clade

Aligned CDS sequences	n. TLP sequences	Π^A	Tajima’s Test (D)	SLAC method^B
Aligned CDS sequences	n. TLP sequences	Π^A	Tajima’s Test (D)	(ω)	PSCs (n.)	NSCs (n.)
TLP clade 1	9	0.070 (0.209)^C	-1.035	0.411	0	3
TLP clade 2	5	0.054 (0.006)	-0.464	0.507	0	0
TLP clade 3	13	0.152 (0.033)	1.091	0.303	0	19
TLP clade 4	6	0.071 (0.024)	-1.226	0.552	0	0
C. americana TLP family	8	0.130 (0.018)	0.152	0.286	0	13
C. avellana TLP family	15	0.257 (0.034)	-0.599	0.388	0	24
C. heterophylla TLP family	5	0.167 (0.013)	0.201	0.296	0	6
C. mandshurica TLP family	5	0.187 (0.011)	0.372	0.289	0	10

In the table are reported: number of TLP sequences, the average evolutionary divergence (π) and the direction and magnitude of natural selection for each analyzed Cor a TLP gene family and phylogentic clade
^AIn brackets was reported the standard error estimate by a bootstrap procedure (100 replicates)
^BNumber of positive (PSCs: positively selected codon sites) and negative (NSCs: negatively selected codon sites) codons statistically significant (P < 0.1) in TLP protein-coding genes
^CPoorly aligned sites were removed using GBlocks

ISSN: 1471-2229